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Palmyra is a village in Jefferson County, Wisconsin, strung along the Scuppernong River next to the Town of Palmyra.

The village was named after Palmyra, Syria — the Powers brothers, who built a sawmill there, chose the name because of the abundance of sand they found in the area, likening it to the Syrian desert city.

Palmyra's first settler, Cyrus Norton, arrived in the Scuppernong Valley in 1839, and the Powers brothers' sawmill opened on Christmas Day 1842; John Fish surveyed and platted the village into lots in 1843.

The railroad reached Palmyra in the fall of 1852, and by 1860 the settlement had grown to roughly 1,000 residents.

Palmyra first incorporated as a village on April 4, 1866, but an error in the act dissolved the charter in 1872; a new charter was granted on May 5, 1874.

The 2020 census counted 1,719 people in the Village of Palmyra, down slightly from 1,781 in 2010; the village covers 1.25 square miles.

In the late 1800s Palmyra was a noted mineral springs resort: the Palmyra Springs Sanitarium was established in 1870 on what is now Lower Spring Lake.

Ira Bidwell of St. Paul developed the springs and in 1874 built the four-story Bidwell House, whose six springs and lake frontage drew 'water cure' patrons from across the state, region and country for about fifty years.

Palmyra's springs industry faded by the 1920s and the old sanitarium building was eventually razed, but the spring lakes remain a feature of the village landscape.

Dairying was another early mainstay around Palmyra: by the 1860s–70s the town had three cheese factories with the combined capacity to handle the milk of about 1,500 cows.